pBABE hBRM

价格:1000元

联系方式:I47-825O-882O

相关技术服务:质粒构建    基因合成    质粒大提

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载体基本信息

质粒名称: pBABE hBRM 
出品公司: 爱迪基因
目录编号: 1961
存储实验室: Robert Kingston

载体骨架基本信息
载体名称: pBABE-puro
载体抗性: 氨苄霉素
空载体大小: 5200 bp
载体类型: 哺乳细胞表达,逆转录病毒
筛选标记: 嘌呤霉素
高拷贝/低拷贝: 高拷贝
生长条件: 37 ℃

插入基因信息
插入基因名称: BRM
物种来源: 人源
基因又名: BAF190b
标签/融合蛋白: flag (C terminal on insert)
克隆方法: 限制性内切酶
5' 克隆位点: EcoRI(未破坏)
3' 克隆位点: EcoRI (未破坏)
5' 测序引物 pBABE 5'

文献引用方法
材料和方法部分: pBABE hBRM was a gift from Robert Kingston (爱迪基因 plasmid # 1961)
参考文献部分: Purification and characterization of mSin3A-containing Brg1 and hBrm chromatin remodeling complexes. Sif S, Saurin AJ, Imbalzano AN, Kingston RE. Genes Dev 2001 Mar 1;15(5):603-18. 10.1101/gad.872801 PubMed 11238380

载体图谱质粒图谱和多克隆位点信息

pBABE hBRM质粒图谱

载体简介

载体序列

LOCUS       pBABE hBRM               10054 bp ds-DNA     circular SYN 10-JAN-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pBABE hBRM
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10054)
  AUTHORS   Sif S, Saurin AJ, Imbalzano AN, Kingston RE
  TITLE     Purification and characterization of mSin3A-containing Brg1 and hBrm
            chromatin remodeling complexes.
  JOURNAL   Genes Dev 2001 Mar 1;15(5):603-18.
  PUBMED    11238380
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|biofeng.com|
COMMENT     VNTNAME|pBABE hBRM|
FEATURES             Location/Qualifiers
     source          1..10054
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             1..417
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     347..369
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     385..401
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             5384..5407
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(5443..5463)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5448..5777
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5628..5763
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5690..5709
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5787..6386
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5787..5806)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6283..6303
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6607..7076
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(7206..7223)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7377..7965)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7457..7476)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8125..8985)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8748..8767
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8986..9090)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             9121..9590
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    9655..9854
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
ORIGIN
        1 ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga tgtcgagcgg
       61 atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt ctgctctgca
      121 gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg agacctcatc
      181 acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga ccaggtcccc
      241 tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa gccctttgta
      301 caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct tgaacctcct
      361 cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct aggcgccggc
      421 cggatcccag tgtggtggta cgtaggaatt ccggatgctc agatgaaagc cccgagatca
      481 cagagacccg gcgagatcac agagacccgg cctgaaggaa cgtggaaaga ccaatgtacc
      541 tgttttgacc ggttgcctgg agcaagaagt tccagttggg gagaattttc agaagataaa
      601 gtcggagatt gtggaaagac ttgacttgca gcattactct actgactggc agagacagga
      661 gaggtagatg tcaacgccca cagaccctgg tgcgatgccc cacccagggc cttcgccggg
      721 gcctgggcct tcccctgggc caattcttgg gcctagtcca ggaccaggac catccccagg
      781 ttccgtccac agcatgatgg ggccaagtcc tggacctcca agtgtctccc atcctatgcc
      841 gacgatgggg tccacagact tcccacagga aggcatgcat caaatgcata agcccatcga
      901 tggtatacat gacaagggga ttgtagaaga catccattgt ggatccatga agggcactgg
      961 tatgcgacca cctcacccag gcatgggccc tccccagagt ccaatggatc aacacagcca
     1021 aggttatatg tcaccacacc catctccatt aggagcccca gagcacgtct ccagccctat
     1081 gtctggagga ggcccaactc cacctcagat gccaccaagc cagccggggg ccctcatccc
     1141 aggtgatccg caggccatga gccagcccaa cagaggtccc tcacctttca gtcctgtcca
     1201 gctgcatcag cttcgagctc agattttagc ttataaaatg ctggcccgag gccagcccct
     1261 ccccgaaacg ctgcagcttg cagtccaggg gaaaaggacg ttgcctggct tgcagcaaca
     1321 acagcagcag caacagcagc agcagcagca gcagcagcag cagcagcagc agcaacagca
     1381 gccgcagcag cagccgccgc aaccacagac gcagcaacaa cagcagccgg cccttgttaa
     1441 ctacaacaga ccatctggcc cggggccgga gctgagcggc ccgagcaccc cgcagaagct
     1501 gccggtgccc gcgcccggcg gccggccctc gcccgcgccc cccgcagccg cgcagccgcc
     1561 cgcggccgca gtgcccgggc cctcagtgcc gcagccggcc ccggggcagc cctcgcccgt
     1621 cctccagctg cagcagaagc agagccgcat cagccccatc cagaaaccgc aaggcctgga
     1681 ccccgtggaa attctgcaag agcgggaata cagacttcag gcccgcatag ctcataggat
     1741 acaagaactg gaaaatctgc ctggctcttt gccaccagat ttaagaacca aagcaaccgt
     1801 ggaactaaaa gcacttcggt tactcaattt ccagcgtcag ctgagacagg aggtggtggc
     1861 ctgcatgcgc agggacacga ccctggagac ggctctcaac tccaaagcat acaaacggag
     1921 caagcgccag actctgagag aagctcgcat gaccgagaag ctggagaagc agcagaagat
     1981 tgagcaggag aggaaacgcc gtcagaaaca ccaggaatac ctgaacagta ttttgcaaca
     2041 tgcaaaagat tttaaggaat atcatcggtc tgtggccgga aagatccaga agctctccaa
     2101 agcagtggca acttggcatg ccaacactga aagagagcag aagaaggaga cagagcggat
     2161 tgaaaaggag agaatgcggc gactgatggc tgaagatgag gagggttata gaaaactgat
     2221 tgatcaaaag aaagacaggc gtttagctta ccttttgcag cagaccgatg agtatgtagc
     2281 caatctgacc aatctggttt gggagcacaa gcaagcccag gcagccaaag agaagaagaa
     2341 gaggaggagg aggaagaaga aggctgagga gaatgcagag ggtggggagt ctgccctggg
     2401 accggatgga gagcccatag atgagagcag ccagatgagt gacctccctg tcaaagtgac
     2461 tcacacagaa accggcaagg ttctgttcgg accagaagca cccaaagcaa gtcagctgga
     2521 cgcctggctg gaaatgaatc ctggttatga agttgcccct agatctgaca gtgaagagag
     2581 tgattctgat tatgaggaag aggatgagga agaagagtcc agtaggcagg aaaccgaaga
     2641 gaaaatactc ctggatccaa atagcgaaga agtttctgag aaggatgcta agcagatcat
     2701 tgagacagct aagcaagacg tggatgatga atacagcatg cagtacagtg ccaggggctc
     2761 ccagtcctac tacaccgtgg ctcatgccat ctcggagagg gtggagaaac agtctgccct
     2821 cctaattaat gggaccctaa agcattacca gctccagggc ctggaatgga tggtttccct
     2881 gtataataac aacttgaacg gaatcttagc cgatgaaatg gggcttggaa agaccataca
     2941 gaccattgca ctcatcactt atctgatgga gcacaaaaga ctcaatggcc cctatctcat
     3001 cattgttccc ctttcgactc tatctaactg gacatatgaa tttgacaaat gggctccttc
     3061 tgtggtgaag atttcttaca agggtactcc tgccatgcgt cgctcccttg tcccccagct
     3121 acggagtggc aaattcaatg tcctcttgac tacttatgag tatattataa aagacaagca
     3181 cattcttgca aagattcggt ggaaatacat gatagtggac gaaggccacc gaatgaagaa
     3241 tcaccactgc aagctgactc aggtcttgaa cactcactat gtggccccca gaaggatcct
     3301 cttgactggg accccgctgc agaataagct ccctgaactc tgggccctcc tcaacttcct
     3361 cctcccaaca atttttaaga gctgcagcac atttgaacaa tggttcaatg ctccatttgc
     3421 catgactggt gaaagggtgg acttaaatga agaagaaact atattgatca tcaggcgtct
     3481 acataaggtg ttaagaccat ttttactaag gagactgaag aaagaagttg aatcccagct
     3541 tcccgaaaaa gtggaatatg tgatcaagtg tgacatgtca gctctgcaga agattctgta
     3601 tcgccatatg caagccaagg ggatccttct cacagatggt tctgagaaag ataagaaggg
     3661 gaaaggaggt gctaagacac ttatgaacac tattatgcag ttgagaaaaa tctgcaacca
     3721 cccatatatg tttcagcaca ttgaggaatc ctttgctgaa cacctaggct attcaaatgg
     3781 ggtcatcaat ggggctgaac tgtatcgggc ctcagggaag tttgagctgc ttgatcgtat
     3841 tctgccaaaa ttgagagcga ctaatcaccg agtgctgctt ttctgccaga tgacatctct
     3901 catgaccatc atggaggatt attttgcttt tcggaacttc ctttacctac gccttgatgg
     3961 caccaccaag tctgaagatc gtgctgcttt gctgaagaaa ttcaatgaac ctggatccca
     4021 gtatttcatt ttcttgctga gcacaagagc tggtggcctg ggcttaaatc ttcaggcagc
     4081 tgatacagtg gtcatctttg acagcgactg gaatcctcat caggatctgc aggcccaaga
     4141 ccgagctcac cgcatcgggc agcagaacga ggtccgggta ctgaggctct gtaccgtgaa
     4201 cagcgtggag gaaaagatcc tcgcggccgc aaaatacaag ctgaacgtgg atcagaaagt
     4261 gatccaggcg ggcatgtttg accaaaagtc ttcaagccac gagcggaggg cattcctgca
     4321 ggccatcttg gagcatgaag aggaaaatga ggaagaagat gaagtaccgg acgatgagac
     4381 tctgaaccaa atgattgctc gacgagaaga agaatttgac ctttttatgc ggatggacat
     4441 ggaccggcgg agggaagatg cccggaaccc gaaacggaag ccccgtttaa tggaggagga
     4501 tgagctgccc tcctggatca ttaaggatga cgctgaagta gaaaggctca cctgtgaaga
     4561 agaggaggag aaaatatttg ggagggggtc ccgccagcgc cgtgacgtgg actacagtga
     4621 cgccctcacg gagaagcagt ggctaagggc catcgaagac ggcaatttgg aggaaatgga
     4681 agaggaagta cggcttaaga agcgaaaaag acgaagaaat gtggataaag atcctgcaaa
     4741 agaagatgtg gaaaaagcta agaagagaag aggccgccct cccgctgaga aactgtcacc
     4801 aaatcccccc aaactgacaa agcagatgaa cgctatcatc gatactgtga taaactacaa
     4861 agatagttca gggcgacagc tcagtgaagt cttcattcag ttaccttcaa ggaaagaatt
     4921 accagaatac tatgaattaa ttaggaagcc agtggatttc aaaaaaataa aggaaaggat
     4981 tcgtaatcat aagtaccgga gcctaggcga cctggagaag gatgtcatgc ttctctgtca
     5041 caacgctcag acgttcaacc tggagggatc ccagatctat gaagactcca tcgtcttaca
     5101 gtcagtgttt aagagtgccc ggcagaaaat tgccaaagag gaagagagtg aggatgaaag
     5161 caatgaagag gaggaagagg aagatgaaga agagtcagag tccgaggcaa aatcagtcaa
     5221 ggtgaaaatt aagctcaata aaaaagatga caaaggccgg gacaaaggga aaggcaagaa
     5281 aaggccaaat cgaggaaaag ccaaacctgt agtgagcgat tttgacagcg atgaggagca
     5341 ggatgaacgt gaacagtcag aaggaagtgg gacggatgat gaggactaca aggacgacga
     5401 tgacaaatga taagaattcg ccagcacagt ggtcgaccct gtggaatgtg tgtcagttag
     5461 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     5521 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     5581 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     5641 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     5701 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     5761 gcctaggctt ttgcaaaaag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca
     5821 cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg
     5881 ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac
     5941 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg
     6001 cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg
     6061 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc
     6121 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc
     6181 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg
     6241 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg
     6301 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt
     6361 gcatgacccg caagcccggt gcctgacgcc cgccccacga cccgcagcgc ccgaccgaaa
     6421 ggagcgcacg accccatgca tcgataaaat aaaagatttt atttagtctc cagaaaaagg
     6481 ggggaatgaa agaccccacc tgtaggtttg gcaagctagc ttaagtaacg ccattttgca
     6541 aggcatggaa aaatacataa ctgagaatag agaagttcag atcaaggtca ggaacagatg
     6601 gaacagctga atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag
     6661 ggccaagaac agatggaaca gctgaatatg ggccaaacag gatatctgtg gtaagcagtt
     6721 cctgccccgg ctcagggcca agaacagatg gtccccagat gcggtccagc cctcagcagt
     6781 ttctagagaa ccatcagatg tttccagggt gccccaagga cctgaaatga ccctgtgcct
     6841 tatttgaact aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct gctccccgag
     6901 ctcaataaaa gagcccacaa cccctcactc ggggcgccag tcctccgatt gactgagtcg
     6961 cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc atccgacttg tggtctcgct
     7021 gttccttggg agggtctcct ctgagtgatt gactacccgt cagcgggggt ctttcacatg
     7081 cagcatgtat caaaattaat ttggtttttt ttcttaagta tttacattaa atggccatag
     7141 ttgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     7201 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     7261 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     7321 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     7381 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     7441 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     7501 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     7561 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     7621 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     7681 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     7741 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     7801 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     7861 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     7921 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     7981 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     8041 agattatcaa aaaggatctt cacctagatc cttttgcggc cgcaaatcaa tctaaagtat
     8101 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     8161 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     8221 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     8281 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     8341 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     8401 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     8461 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     8521 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     8581 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     8641 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     8701 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     8761 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     8821 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     8881 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     8941 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     9001 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     9061 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgcagc
     9121 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
     9181 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
     9241 ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag
     9301 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg
     9361 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat
     9421 aaaagagccc acaacccctc actcggggcg ccagtcctcc gattgactga gtcgcccggg
     9481 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct
     9541 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca tttgggggct
     9601 cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg aggcaagctg
     9661 gccagcaact tatctgtgtc tgtccgattg tctagtgtct atgactgatt ttatgcgcct
     9721 gcgtcggtac tagttagcta actagctctg tatctggcgg acccgtggtg gaactgacga
     9781 gttctgaaca cccggccgca accctgggag acgtcccagg gactttgggg gccgtttttg
     9841 tggcccgacc tgaggaaggg agtcgatgtg gaatccgacc ccgtcaggat atgtggttct
     9901 ggtaggagac gagaacctaa aacagttccc gcctccgtct gaatttttgc tttcggtttg
     9961 gaaccgaagc cgcgcgtctt gtctgctgca gcgctgcagc atcgttctgt gttgtctctg
    10021 tctgactgtg tttctgtatt tgtctgaaaa ttag
//

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