质粒名称: | pBABE hBRM |
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出品公司: | 爱迪基因 |
目录编号: | 1961 |
存储实验室: | Robert Kingston |
载体骨架基本信息 |
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载体名称: | pBABE-puro |
载体抗性: | 氨苄霉素 |
空载体大小: | 5200 bp |
载体类型: | 哺乳细胞表达,逆转录病毒 |
筛选标记: | 嘌呤霉素 |
高拷贝/低拷贝: | 高拷贝 |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | BRM |
物种来源: | 人源 |
基因又名: | BAF190b |
标签/融合蛋白: | flag (C terminal on insert) |
克隆方法: | 限制性内切酶 |
5' 克隆位点: | EcoRI(未破坏) |
3' 克隆位点: | EcoRI (未破坏) |
5' 测序引物 | pBABE 5' |
文献引用方法 |
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材料和方法部分: | pBABE hBRM was a gift from Robert Kingston (爱迪基因 plasmid # 1961) |
参考文献部分: | Purification and characterization of mSin3A-containing Brg1 and hBrm chromatin remodeling complexes. Sif S, Saurin AJ, Imbalzano AN, Kingston RE. Genes Dev 2001 Mar 1;15(5):603-18. 10.1101/gad.872801 PubMed 11238380 |
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载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pBABE hBRM 10054 bp ds-DNA circular SYN 10-JAN-2018 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pBABE hBRM SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10054) AUTHORS Sif S, Saurin AJ, Imbalzano AN, Kingston RE TITLE Purification and characterization of mSin3A-containing Brg1 and hBrm chromatin remodeling complexes. JOURNAL Genes Dev 2001 Mar 1;15(5):603-18. PUBMED 11238380 COMMENT This file is created by Vector NTI http://www.biofeng.com/ COMMENT ORIGDB|GenBank COMMENT VNTAUTHORNAME|biofeng.com| COMMENT VNTNAME|pBABE hBRM| FEATURES Location/Qualifiers source 1..10054 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 1..417 /label=gag (truncated) /note="truncated Moloney murine leukemia virus (MMLV) gag gene lacking the start codon" primer_bind 347..369 /label=pLXSN 5' /note="Murine stem cell virus, forward primer. Also called MSCV" primer_bind 385..401 /label=pBABE 5' /note="Psi packaging signal, forward primer for pBABE vectors" CDS 5384..5407 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" primer_bind complement(5443..5463) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 5448..5777 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 5628..5763 /label=SV40 ori /note="SV40 origin of replication" primer_bind 5690..5709 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 5787..6386 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(5787..5806) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 6283..6303 /label=Puro-F /note="Puromycin resistance gene, forward primer" LTR 6607..7076 /note="long terminal repeat from Moloney murine leukemia virus" primer_bind complement(7206..7223) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(7377..7965) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(7457..7476) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(8125..8985) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 8748..8767 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(8986..9090) /gene="bla" /label=AmpR promoter LTR 9121..9590 /note="long terminal repeat from Moloney murine leukemia virus" misc_feature 9655..9854 /label=MMLV Psi /note="packaging signal of Moloney murine leukemia virus (MMLV)" ORIGIN 1 ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga tgtcgagcgg 61 atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt ctgctctgca 121 gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg agacctcatc 181 acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga ccaggtcccc 241 tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa gccctttgta 301 caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct tgaacctcct 361 cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct aggcgccggc 421 cggatcccag tgtggtggta cgtaggaatt ccggatgctc agatgaaagc cccgagatca 481 cagagacccg gcgagatcac agagacccgg cctgaaggaa cgtggaaaga ccaatgtacc 541 tgttttgacc ggttgcctgg agcaagaagt tccagttggg gagaattttc agaagataaa 601 gtcggagatt gtggaaagac ttgacttgca gcattactct actgactggc agagacagga 661 gaggtagatg tcaacgccca cagaccctgg tgcgatgccc cacccagggc cttcgccggg 721 gcctgggcct tcccctgggc caattcttgg gcctagtcca ggaccaggac catccccagg 781 ttccgtccac agcatgatgg ggccaagtcc tggacctcca agtgtctccc atcctatgcc 841 gacgatgggg tccacagact tcccacagga aggcatgcat caaatgcata agcccatcga 901 tggtatacat gacaagggga ttgtagaaga catccattgt ggatccatga agggcactgg 961 tatgcgacca cctcacccag gcatgggccc tccccagagt ccaatggatc aacacagcca 1021 aggttatatg tcaccacacc catctccatt aggagcccca gagcacgtct ccagccctat 1081 gtctggagga ggcccaactc cacctcagat gccaccaagc cagccggggg ccctcatccc 1141 aggtgatccg caggccatga gccagcccaa cagaggtccc tcacctttca gtcctgtcca 1201 gctgcatcag cttcgagctc agattttagc ttataaaatg ctggcccgag gccagcccct 1261 ccccgaaacg ctgcagcttg cagtccaggg gaaaaggacg ttgcctggct tgcagcaaca 1321 acagcagcag caacagcagc agcagcagca gcagcagcag cagcagcagc agcaacagca 1381 gccgcagcag cagccgccgc aaccacagac gcagcaacaa cagcagccgg cccttgttaa 1441 ctacaacaga ccatctggcc cggggccgga gctgagcggc ccgagcaccc cgcagaagct 1501 gccggtgccc gcgcccggcg gccggccctc gcccgcgccc cccgcagccg cgcagccgcc 1561 cgcggccgca gtgcccgggc cctcagtgcc gcagccggcc ccggggcagc cctcgcccgt 1621 cctccagctg cagcagaagc agagccgcat cagccccatc cagaaaccgc aaggcctgga 1681 ccccgtggaa attctgcaag agcgggaata cagacttcag gcccgcatag ctcataggat 1741 acaagaactg gaaaatctgc ctggctcttt gccaccagat ttaagaacca aagcaaccgt 1801 ggaactaaaa gcacttcggt tactcaattt ccagcgtcag ctgagacagg aggtggtggc 1861 ctgcatgcgc agggacacga ccctggagac ggctctcaac tccaaagcat acaaacggag 1921 caagcgccag actctgagag aagctcgcat gaccgagaag ctggagaagc agcagaagat 1981 tgagcaggag aggaaacgcc gtcagaaaca ccaggaatac ctgaacagta ttttgcaaca 2041 tgcaaaagat tttaaggaat atcatcggtc tgtggccgga aagatccaga agctctccaa 2101 agcagtggca acttggcatg ccaacactga aagagagcag aagaaggaga cagagcggat 2161 tgaaaaggag agaatgcggc gactgatggc tgaagatgag gagggttata gaaaactgat 2221 tgatcaaaag aaagacaggc gtttagctta ccttttgcag cagaccgatg agtatgtagc 2281 caatctgacc aatctggttt gggagcacaa gcaagcccag gcagccaaag agaagaagaa 2341 gaggaggagg aggaagaaga aggctgagga gaatgcagag ggtggggagt ctgccctggg 2401 accggatgga gagcccatag atgagagcag ccagatgagt gacctccctg tcaaagtgac 2461 tcacacagaa accggcaagg ttctgttcgg accagaagca cccaaagcaa gtcagctgga 2521 cgcctggctg gaaatgaatc ctggttatga agttgcccct agatctgaca gtgaagagag 2581 tgattctgat tatgaggaag aggatgagga agaagagtcc agtaggcagg aaaccgaaga 2641 gaaaatactc ctggatccaa atagcgaaga agtttctgag aaggatgcta agcagatcat 2701 tgagacagct aagcaagacg tggatgatga atacagcatg cagtacagtg ccaggggctc 2761 ccagtcctac tacaccgtgg ctcatgccat ctcggagagg gtggagaaac agtctgccct 2821 cctaattaat gggaccctaa agcattacca gctccagggc ctggaatgga tggtttccct 2881 gtataataac aacttgaacg gaatcttagc cgatgaaatg gggcttggaa agaccataca 2941 gaccattgca ctcatcactt atctgatgga gcacaaaaga ctcaatggcc cctatctcat 3001 cattgttccc ctttcgactc tatctaactg gacatatgaa tttgacaaat gggctccttc 3061 tgtggtgaag atttcttaca agggtactcc tgccatgcgt cgctcccttg tcccccagct 3121 acggagtggc aaattcaatg tcctcttgac tacttatgag tatattataa aagacaagca 3181 cattcttgca aagattcggt ggaaatacat gatagtggac gaaggccacc gaatgaagaa 3241 tcaccactgc aagctgactc aggtcttgaa cactcactat gtggccccca gaaggatcct 3301 cttgactggg accccgctgc agaataagct ccctgaactc tgggccctcc tcaacttcct 3361 cctcccaaca atttttaaga gctgcagcac atttgaacaa tggttcaatg ctccatttgc 3421 catgactggt gaaagggtgg acttaaatga agaagaaact atattgatca tcaggcgtct 3481 acataaggtg ttaagaccat ttttactaag gagactgaag aaagaagttg aatcccagct 3541 tcccgaaaaa gtggaatatg tgatcaagtg tgacatgtca gctctgcaga agattctgta 3601 tcgccatatg caagccaagg ggatccttct cacagatggt tctgagaaag ataagaaggg 3661 gaaaggaggt gctaagacac ttatgaacac tattatgcag ttgagaaaaa tctgcaacca 3721 cccatatatg tttcagcaca ttgaggaatc ctttgctgaa cacctaggct attcaaatgg 3781 ggtcatcaat ggggctgaac tgtatcgggc ctcagggaag tttgagctgc ttgatcgtat 3841 tctgccaaaa ttgagagcga ctaatcaccg agtgctgctt ttctgccaga tgacatctct 3901 catgaccatc atggaggatt attttgcttt tcggaacttc ctttacctac gccttgatgg 3961 caccaccaag tctgaagatc gtgctgcttt gctgaagaaa ttcaatgaac ctggatccca 4021 gtatttcatt ttcttgctga gcacaagagc tggtggcctg ggcttaaatc ttcaggcagc 4081 tgatacagtg gtcatctttg acagcgactg gaatcctcat caggatctgc aggcccaaga 4141 ccgagctcac cgcatcgggc agcagaacga ggtccgggta ctgaggctct gtaccgtgaa 4201 cagcgtggag gaaaagatcc tcgcggccgc aaaatacaag ctgaacgtgg atcagaaagt 4261 gatccaggcg ggcatgtttg accaaaagtc ttcaagccac gagcggaggg cattcctgca 4321 ggccatcttg gagcatgaag aggaaaatga ggaagaagat gaagtaccgg acgatgagac 4381 tctgaaccaa atgattgctc gacgagaaga agaatttgac ctttttatgc ggatggacat 4441 ggaccggcgg agggaagatg cccggaaccc gaaacggaag ccccgtttaa tggaggagga 4501 tgagctgccc tcctggatca ttaaggatga cgctgaagta gaaaggctca cctgtgaaga 4561 agaggaggag aaaatatttg ggagggggtc ccgccagcgc cgtgacgtgg actacagtga 4621 cgccctcacg gagaagcagt ggctaagggc catcgaagac ggcaatttgg aggaaatgga 4681 agaggaagta cggcttaaga agcgaaaaag acgaagaaat gtggataaag atcctgcaaa 4741 agaagatgtg gaaaaagcta agaagagaag aggccgccct cccgctgaga aactgtcacc 4801 aaatcccccc aaactgacaa agcagatgaa cgctatcatc gatactgtga taaactacaa 4861 agatagttca gggcgacagc tcagtgaagt cttcattcag ttaccttcaa ggaaagaatt 4921 accagaatac tatgaattaa ttaggaagcc agtggatttc aaaaaaataa aggaaaggat 4981 tcgtaatcat aagtaccgga gcctaggcga cctggagaag gatgtcatgc ttctctgtca 5041 caacgctcag acgttcaacc tggagggatc ccagatctat gaagactcca tcgtcttaca 5101 gtcagtgttt aagagtgccc ggcagaaaat tgccaaagag gaagagagtg aggatgaaag 5161 caatgaagag gaggaagagg aagatgaaga agagtcagag tccgaggcaa aatcagtcaa 5221 ggtgaaaatt aagctcaata aaaaagatga caaaggccgg gacaaaggga aaggcaagaa 5281 aaggccaaat cgaggaaaag ccaaacctgt agtgagcgat tttgacagcg atgaggagca 5341 ggatgaacgt gaacagtcag aaggaagtgg gacggatgat gaggactaca aggacgacga 5401 tgacaaatga taagaattcg ccagcacagt ggtcgaccct gtggaatgtg tgtcagttag 5461 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt 5521 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca 5581 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa 5641 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag 5701 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag 5761 gcctaggctt ttgcaaaaag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca 5821 cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg 5881 ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac 5941 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg 6001 cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg 6061 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc 6121 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc 6181 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg 6241 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg 6301 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt 6361 gcatgacccg caagcccggt gcctgacgcc cgccccacga cccgcagcgc ccgaccgaaa 6421 ggagcgcacg accccatgca tcgataaaat aaaagatttt atttagtctc cagaaaaagg 6481 ggggaatgaa agaccccacc tgtaggtttg gcaagctagc ttaagtaacg ccattttgca 6541 aggcatggaa aaatacataa ctgagaatag agaagttcag atcaaggtca ggaacagatg 6601 gaacagctga atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag 6661 ggccaagaac agatggaaca gctgaatatg ggccaaacag gatatctgtg gtaagcagtt 6721 cctgccccgg ctcagggcca agaacagatg gtccccagat gcggtccagc cctcagcagt 6781 ttctagagaa ccatcagatg tttccagggt gccccaagga cctgaaatga ccctgtgcct 6841 tatttgaact aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct gctccccgag 6901 ctcaataaaa gagcccacaa cccctcactc ggggcgccag tcctccgatt gactgagtcg 6961 cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc atccgacttg tggtctcgct 7021 gttccttggg agggtctcct ctgagtgatt gactacccgt cagcgggggt ctttcacatg 7081 cagcatgtat caaaattaat ttggtttttt ttcttaagta tttacattaa atggccatag 7141 ttgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc 7201 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct 7261 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg 7321 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc 7381 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga 7441 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct 7501 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg 7561 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag 7621 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat 7681 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac 7741 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac 7801 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc 7861 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt 7921 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc 7981 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg 8041 agattatcaa aaaggatctt cacctagatc cttttgcggc cgcaaatcaa tctaaagtat 8101 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc 8161 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat 8221 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc 8281 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 8341 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag 8401 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg 8461 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg 8521 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag 8581 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt 8641 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga 8701 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc 8761 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc 8821 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc 8881 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc 8941 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca 9001 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 9061 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgcagc 9121 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa 9181 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc 9241 ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag 9301 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg 9361 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat 9421 aaaagagccc acaacccctc actcggggcg ccagtcctcc gattgactga gtcgcccggg 9481 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct 9541 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca tttgggggct 9601 cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg aggcaagctg 9661 gccagcaact tatctgtgtc tgtccgattg tctagtgtct atgactgatt ttatgcgcct 9721 gcgtcggtac tagttagcta actagctctg tatctggcgg acccgtggtg gaactgacga 9781 gttctgaaca cccggccgca accctgggag acgtcccagg gactttgggg gccgtttttg 9841 tggcccgacc tgaggaaggg agtcgatgtg gaatccgacc ccgtcaggat atgtggttct 9901 ggtaggagac gagaacctaa aacagttccc gcctccgtct gaatttttgc tttcggtttg 9961 gaaccgaagc cgcgcgtctt gtctgctgca gcgctgcagc atcgttctgt gttgtctctg 10021 tctgactgtg tttctgtatt tgtctgaaaa ttag //
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